Metabolism
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Overheating after overdosing on E - and slimming by taking dinitrophenol
Objectives
By the end of this exercise you should be able to:
Describe the process of oxidation of metabolic fuels to carbon dioxide and water linked to the phosphorylation of ADP to ATP
Relate the obligatory linkage of phosphorylation of ADP to ATP to the oxidation of coenzymes and reduction of oxygen to water
Explain why some substrates have a P:O ratio of ~2.5 and others of ~1.5
Name the different types of coenzymes in the mitochondrial electron transport chain and differentiate between electron carriers and hydrogen carriers
Explain why the overall process of oxidative phosphorylation requires intact mitochondrial membrane vesicles
Explain how electron transport creates a proton gradient across the mitochondrial inner membrane and how this is coupled to the phosphorylation of ADP to ATP
Explain how physical activity controls the rate at which metabolic fuels are oxidised
Explain the actions of uncouplers and the biochemical basis of non-shivering thermogenesis.